Background: Health promotion lifestyle is a multidimensional pattern of self-initiated actions and perceptions that serve to maintain and enhance the level of wellness, self-actualization and fulfillment of the individual. Objective: The main purpose of the present study was to investigate psychometric properties of the Health-Promoting Lifestyle Profile-II (HPLP-II, Walker, Sechrist & Pender, 1995) among Iranian university students. Method: 324 university students (130 male, 194 female) completed the HPLP-II and the Emotional Adjustment Measure (EAM, Rubio, Aguado, Hontangas & Hernandez, 2007). The confirmatory factor analysis method and internal consistency were used to compute the HPLP-II's factorial validity and reliability, respectively. Also, in order to examine the construct validity of the HPLP-II, we computed correlations between different dimensions of HPLP-II with emotional adjustment. Results: The results of confirmatory factor analysis indicated that 6-factor structure of the HPLP-II consisted health responsibility, physical activity, nutrition, spiritual growth, interpersonal relations and stress management had good fit to data in the Iranian sample. Correlational analyses between different dimensions of HPLP-II with emotional adjustment provided initial evidence for the PSRS convergent validity. Cronbach’s α Coefficients ranged from 0/56 to 0/80 for six dimensions. Conclusion: In sum, these findings provide evidence for the validity and reliability of the HPLP-II as an instrument to measure of health lifestyle among Iranian university students.