The Description of lifestyle based on Islamic beliefs is one of the most important needs of Islamic society. Therefore this research has been conducted to investigate the validation the Islamic lifestyle questionnaire for couples (ILSQ). The method of this research is based on combination type and consists of two stages. The first stage is qualitative method for making essential tools. For this purpose, in addition to interview with experts in the field of family sciences and Islamic sciences; extensive library studies was performed and theoretical model of Islamic lifestyle for couples was developed and the questionnaire was made. In the second stage of this research, validity and reliability of this tool was examined by a connotative method. So 150  student’s parents of Isfahan university students  was selected by cluster sampling, And they complete the researcher build questionnaire (ILSQ) and short form of spanier couple adjustment questionnaire. The data analyzed by descriptive and inferential statistical method in SPSS and Amos software.The findings showed that lifestyle questionnaire contains 84 questions and eight dimensions: structural, spiritual, behavioral, emotional, cognitive, sexual, relationships with families and healthcare. Calculating the reliability and validity of the questionnaire, was shown that the (ILSQ)has high reliability and validity. Thus, it can be used in researches and evaluations.